Journal: Materials Today Bio

Article Title: Orchestrating the immuno-chondrogenic microenvironment via a bio-inorganic hybrid microsphere system for tendon-to-bone healing

doi: 10.1016/j.mtbio.2026.102851

Figure Lengend Snippet: ZIF-8/sEVs alleviate the inhibitory effects of the M1 M ϕ -mediated inflammatory microenvironment on BMSC migration and chondrogenic differentiation. A) Representative immunofluorescence images of the chemotaxis-associated proteins CXCL12 (green) and CXCR4 (red) in BMSCs cultured under different conditioned media. Nuclei were stained with DAPI (blue). B) Representative optical microscopy images of the scratch wound healing assay at 0 h and 24 h to assess the migratory capacity of BMSCs. C, D) Semi-quantitative analysis of the relative fluorescence intensity of CXCL12 and CXCR4 (C) and the wound healing ratio (D), indicating that ZIF-8/sEVs restore migration inhibited by M1 CM. E–G) Representative immunofluorescence staining of key chondrogenic markers in BMSCs, including (E) Type II Collagen (COL II, red), (F) Aggrecan (red), and (G) Sox-9 (red). The cytoskeleton was stained with Phalloidin (green) and nuclei with DAPI (blue). H–J) Semi-quantitative analysis of the relative fluorescence intensity for (H) COL II, (I) Aggrecan, and (J) Sox-9. M1 CM: M1 M ϕ conditioned media. n = 3. ∗∗p < 0.01, ∗∗∗p < 0.001, ns: p-value with no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Antibodies against HSP70, CD9, CD81, Calnexin, iNOS, CD206, TNF-α, IL-10, CD29, CD90, CD68, CXCL12, CXCR4, COL II, Aggrecan, Sox-9, PI3K, p-PI3K, AKT, p-AKT, and GAPDH were purchased from Abcam (UK), Cell Signaling Technology (USA), Servicebio (Wuhan, China), Abclonal (Wuhan, China), Biolegend (Beijing, China), or Proteintech (Wuhan, China) (detailed information listed in ).

Techniques: Migration, Immunofluorescence, Chemotaxis Assay, Cell Culture, Staining, Microscopy, Wound Healing Assay, Fluorescence

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: Serum CXCL12 is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via ELISA. The data are presented as the means ± SDs, n=20, **** p < 0.0001

Article Snippet: The samples were then used for the quantitative detection of CXCL12 via an ELISA kit (Elabscience, E-EL-H0052, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Apoptosis

Article Title: Spatially defined danger zone shapes gastric cancer progression through CCDC80 + fibroblast–induced CD8 + T cell dysfunction

doi: 10.1007/s10495-026-02287-1

Figure Lengend Snippet: CCDC80 + fibroblasts recruit effector CD8 + T cells into the danger zone and drive their exhaustion via CXCL12. a Spatial deconvolution of effector CD8 + T cells (CD8_eff) and exhausted CD8 + T cells (CD8_exhau). b Spatial CellChat analysis of CXCL signaling between CD8 + T cell subsets and CCDC80 + or CCDC80 − fibroblasts. c Bubble plot of ligand-receptor pairs between CD8 + T cell subsets and CCDC80 + or CCDC80 − fibroblasts. d Ligand–receptor interaction analysis showing exclusive engagement of CCDC80 + fibroblasts in CXCL12–CXCR4 signaling with both CD8_eff and CD8_exhau cells. e Quantification of CD8_eff and CD8_exhau recruitment in intestinal-type GC (GC3). f Spatial clustering of GC6 sample. g Pseudotime trajectory from cluster 1 to cluster 0. h Representative trajectory (15 − 10/38/62) demonstrating spatial progression to exhaustion. i qRT-PCR for CCDC80 mRNA expression in NIH-3T3 fibroblasts. j Immunoblot for CCDC80 protein expression. k qRT-PCR for CXCL12 mRNA in CCDC80-overexpressing fibroblasts. l ELISA for quantification of CXCL12 secretion. m Schematic of fibroblast-CD8 + T cell co-culture experiment. n Flow cytometry for PD-1 expression on CD8 + T cells with or without CXCL12 blockade. o Flow cytometry for TIM-3 expression on CD8 + T cells with or without CXCL12 blockade

Article Snippet: The collected supernatant was assayed for CXCL12 using an ELISA kit (Cat# MCX120, R&D Systems, Minneapolis, MN, USA), following the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Flow Cytometry